Publications

Found 364 results
2006
Lebensohn AM, Ma L, Ho H-YHenry, Kirschner MW.  2006.  Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts.. Methods Enzymol. 406:156-73.
Bourlat SJ, Juliusdottir T, Lowe CJ, Freeman R, Aronowicz J, Kirschner MW, Lander ES, Thorndyke M, Nakano H, Kohn AB et al..  2006.  Deuterostome phylogeny reveals monophyletic chordates and the new phylum Xenoturbellida.. Nature. 444(7115):85-8.
Lowe CJ, Terasaki M, Wu M, Freeman RM, Runft L, Kwan K, Haigo S, Aronowicz J, Lander E, Gruber C et al..  2006.  Dorsoventral patterning in hemichordates: insights into early chordate evolution.. PLoS Biol. 4(9):e291.
Weiner OD, Rentel MC, Ott A, Brown GE, Jedrychowski M, Yaffe MB, Gygi SP, Cantley LC, Bourne HR, Kirschner MW.  2006.  Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis.. PLoS Biol. 4(2):e38.
Ho H-YHenry, Rohatgi R, Lebensohn AM, Kirschner MW.  2006.  In vitro reconstitution of cdc42-mediated actin assembly using purified components.. Methods Enzymol. 406:174-90.
Kirschner MW.  2006.  Obituary: Reinhart Heinrich (1946-2006).. Nature. 444(7120):700.
Steen H, Jebanathirajah JA, Rush J, Morrice N, Kirschner MW.  2006.  Phosphorylation analysis by mass spectrometry: myths, facts, and the consequences for qualitative and quantitative measurements.. Mol Cell Proteomics. 5(1):172-81.
Rape M, Reddy SK, Kirschner MW.  2006.  The processivity of multiubiquitination by the APC determines the order of substrate degradation.. Cell. 124(1):89-103.
2005
Jebanathirajah JA, Pittman JL, Thomson BA, Budnik BA, Kaur P, Rape M, Kirschner MW, Costello CE, O'Connor PB.  2005.  Characterization of a new qQq-FTICR mass spectrometer for post-translational modification analysis and top-down tandem mass spectrometry of whole proteins.. J Am Soc Mass Spectrom. 16(12):1985-99.
Viadiu H, Stemmann O, Kirschner MW, Walz T.  2005.  Domain structure of separase and its binding to securin as determined by EM.. Nat Struct Mol Biol. 12(6):552-3.
Lee LA, Lee E, Anderson MA, Vardy L, Tahinci E, Ali SM, Kashevsky H, Benasutti M, Kirschner MW, Orr-Weaver TL.  2005.  Drosophila genome-scale screen for PAN GU kinase substrates identifies Mat89Bb as a cell cycle regulator.. Dev Cell. 8(3):435-42.
Gerhart J, Lowe C, Kirschner MW.  2005.  Hemichordates and the origin of chordates.. Curr Opin Genet Dev. 15(4):461-7.
Ayad NG, Rankin S, Ooi D, Rape M, Kirschner MW.  2005.  Identification of ubiquitin ligase substrates by in vitro expression cloning.. Methods Enzymol. 399:404-14.
Kirschner MW.  2005.  The meaning of systems biology.. Cell. 121(4):503-4.
Kwan KM, Kirschner MW.  2005.  A microtubule-binding Rho-GEF controls cell morphology during convergent extension of Xenopus laevis.. Development. 132(20):4599-610.
Rankin S, Ayad NG, Kirschner MW.  2005.  Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates.. Mol Cell. 18(2):185-200.
Steen H, Jebanathirajah JA, Springer M, Kirschner MW.  2005.  Stable isotope-free relative and absolute quantitation of protein phosphorylation stoichiometry by MS.. Proc Natl Acad Sci U S A. 102(11):3948-53.
2004
Rape M, Kirschner MW.  2004.  Autonomous regulation of the anaphase-promoting complex couples mitosis to S-phase entry.. Nature. 432(7017):588-95.
Peterson JR, Bickford LC, Morgan D, Kim AS, Ouerfelli O, Kirschner MW, Rosen MK.  2004.  Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation.. Nat Struct Mol Biol. 11(8):747-55.
Campellone KG, Rankin S, Pawson T, Kirschner MW, Tipper DJ, Leong JM.  2004.  Clustering of Nck by a 12-residue Tir phosphopeptide is sufficient to trigger localized actin assembly.. J Cell Biol. 164(3):407-16.
Wei W, Ayad NG, Wan Y, Zhang G-J, Kirschner MW, Kaelin WG.  2004.  Degradation of the SCF component Skp2 in cell-cycle phase G1 by the anaphase-promoting complex.. Nature. 428(6979):194-8.
Martinez-Quiles N, Ho H-YHenry, Kirschner MW, Ramesh N, Geha RS.  2004.  Erk/Src phosphorylation of cortactin acts as a switch on-switch off mechanism that controls its ability to activate N-WASP.. Mol Cell Biol. 24(12):5269-80.
Lowe CJ, Tagawa K, Humphreys T, Kirschner MW, Gerhart J.  2004.  Hemichordate embryos: procurement, culture, and basic methods.. Methods Cell Biol. 74:171-94.
Gautreau A, Ho H-yiH, Li J, Steen H, Gygi SP, Kirschner MW.  2004.  Purification and architecture of the ubiquitous Wave complex.. Proc Natl Acad Sci U S A. 101(13):4379-83.
Margalit DN, Romberg L, Mets RB, Hebert AM, Mitchison TJ, Kirschner MW, RayChaudhuri D.  2004.  Targeting cell division: small-molecule inhibitors of FtsZ GTPase perturb cytokinetic ring assembly and induce bacterial lethality.. Proc Natl Acad Sci U S A. 101(32):11821-6.
Ho H-YHenry, Rohatgi R, Lebensohn AM, Li J, Gygi SP, Kirschner MW.  2004.  Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex.. Cell. 118(2):203-16.
Song MSup, Song SJeong, Ayad NG, Chang JSook, Lee JHyun, Hong HKyung, Lee H, Choi N, Kim J, Kim H et al..  2004.  The tumour suppressor RASSF1A regulates mitosis by inhibiting the APC-Cdc20 complex.. Nat Cell Biol. 6(2):129-37.
2003
Gerber SA, Rush J, Stemman O, Kirschner MW, Gygi SP.  2003.  Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS.. Proc Natl Acad Sci U S A. 100(12):6940-5.
Lowe CJ, Wu M, Salic A, Evans L, Lander E, Stange-Thomann N, Gruber CE, Gerhart J, Kirschner MW.  2003.  Anteroposterior patterning in hemichordates and the origins of the chordate nervous system.. Cell. 113(7):853-65.
Osterfield M, Kirschner MW, Flanagan JG.  2003.  Graded positional information: interpretation for both fate and guidance.. Cell. 113(4):425-8.
Chabes ALena, Pfleger CM, Kirschner MW, Thelander L.  2003.  Mouse ribonucleotide reductase R2 protein: a new target for anaphase-promoting complex-Cdh1-mediated proteolysis.. Proc Natl Acad Sci U S A. 100(7):3925-9.
Lee E, Salic A, Krüger R, Heinrich R, Kirschner MW.  2003.  The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathway.. PLoS Biol. 1(1):E10.
Collavin L, Kirschner MW.  2003.  The secreted Frizzled-related protein Sizzled functions as a negative feedback regulator of extreme ventral mesoderm.. Development. 130(4):805-16.
Ayad NG, Rankin S, Murakami M, Jebanathirajah J, Gygi S, Kirschner MW.  2003.  Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC.. Cell. 113(1):101-13.
Yan C, Martinez-Quiles N, Eden S, Shibata T, Takeshima F, Shinkura R, Fujiwara Y, Bronson R, Snapper SB, Kirschner MW et al..  2003.  WAVE2 deficiency reveals distinct roles in embryogenesis and Rac-mediated actin-based motility.. EMBO J. 22(14):3602-12.
Kwan KM, Kirschner MW.  2003.  Xbra functions as a switch between cell migration and convergent extension in the Xenopus gastrula.. Development. 130(9):1961-72.
2002
Zou H, Stemman O, Anderson JS, Mann M, Kirschner MW.  2002.  Anaphase specific auto-cleavage of separase.. FEBS Lett. 528(1-3):246-50.
Farhana L, Dawson M, Rishi AK, Zhang Y, Van Buren E, Trivedi C, Reichert U, Fang G, Kirschner MW, Fontana JA.  2002.  Cyclin B and E2F-1 expression in prostate carcinoma cells treated with the novel retinoid CD437 are regulated by the ubiquitin-mediated pathway.. Cancer Res. 62(13):3842-9.
Castiglione F, Bernaschi M, Succi S, Heinrich R, Kirschner MW.  2002.  Intracellular signal propagation in a two-dimensional autocatalytic reaction model.. Phys Rev E Stat Nonlin Soft Matter Phys. 66(3 Pt 1):031905.
Eden S, Rohatgi R, Podtelejnikov AV, Mann M, Kirschner MW.  2002.  Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck.. Nature. 418(6899):790-3.
Holmen SL, Salic A, Zylstra CR, Kirschner MW, Williams BO.  2002.  A novel set of Wnt-Frizzled fusion proteins identifies receptor components that activate beta -catenin-dependent signaling.. J Biol Chem. 277(38):34727-35.
Weiner OD, Neilsen PO, Prestwich GD, Kirschner MW, Cantley LC, Bourne HR.  2002.  A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity.. Nat Cell Biol. 4(7):509-13.
Zhu Z, Kirschner MW.  2002.  Regulated proteolysis of Xom mediates dorsoventral pattern formation during early Xenopus development.. Dev Cell. 3(4):557-68.
Georgi AB, P Stukenberg T, Kirschner MW.  2002.  Timing of events in mitosis.. Curr Biol. 12(2):105-14.
2001
Wan Y, Liu X, Kirschner MW.  2001.  The anaphase-promoting complex mediates TGF-beta signaling by targeting SnoN for destruction.. Mol Cell. 8(5):1027-39.
Peterson JR, Lokey RS, Mitchison TJ, Kirschner MW.  2001.  A chemical inhibitor of N-WASP reveals a new mechanism for targeting protein interactions.. Proc Natl Acad Sci U S A. 98(19):10624-9.
Ho HY, Rohatgi R, Ma L, Kirschner MW.  2001.  CR16 forms a complex with N-WASP in brain and is a novel member of a conserved proline-rich actin-binding protein family.. Proc Natl Acad Sci U S A. 98(20):11306-11.
Stemmann O, Zou H, Gerber SA, Gygi SP, Kirschner MW.  2001.  Dual inhibition of sister chromatid separation at metaphase.. Cell. 107(6):715-26.
Wan Y, Kirschner MW.  2001.  Identification of multiple CDH1 homologues in vertebrates conferring different substrate specificities.. Proc Natl Acad Sci U S A. 98(23):13066-71.
Roberts RJ, Varmus HE, Ashburner M, Brown PO, Eisen MB, Khosla C, Kirschner MW, Nusse R, Scott M, Wold B.  2001.  Information access. Building a "GenBank" of the published literature.. Science. 291(5512):2318-9.
Pfleger CM, Salic A, Lee E, Kirschner MW.  2001.  Inhibition of Cdh1-APC by the MAD2-related protein MAD2L2: a novel mechanism for regulating Cdh1.. Genes Dev. 15(14):1759-64.
Davis RL, Turner DL, Evans LM, Kirschner MW.  2001.  Molecular targets of vertebrate segmentation: two mechanisms control segmental expression of Xenopus hairy2 during somite formation.. Dev Cell. 1(4):553-65.
Rohatgi R, Nollau P, Ho HY, Kirschner MW, Mayer BJ.  2001.  Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2/3 pathway.. J Biol Chem. 276(28):26448-52.
Lee E, Salic A, Kirschner MW.  2001.  Physiological regulation of [beta]-catenin stability by Tcf3 and CK1epsilon.. J Cell Biol. 154(5):983-93.
Stukenberg PT, Kirschner MW.  2001.  Pin1 acts catalytically to promote a conformational change in Cdc25.. Mol Cell. 7(5):1071-83.
Pfleger CM, Lee E, Kirschner MW.  2001.  Substrate recognition by the Cdc20 and Cdh1 components of the anaphase-promoting complex.. Genes Dev. 15(18):2396-407.
2000
Chen F, Ma L, Parrini MC, Mao X, Lopez M, Wu C, Marks PW, Davidson L, Kwiatkowski DJ, Kirchhausen T et al..  2000.  Cdc42 is required for PIP(2)-induced actin polymerization and early development but not for cell viability.. Curr Biol. 10(13):758-65.
Salic A, Lee E, Mayer L, Kirschner MW.  2000.  Control of beta-catenin stability: reconstitution of the cytoplasmic steps of the wnt pathway in Xenopus egg extracts.. Mol Cell. 5(3):523-32.
Davis RL, Kirschner MW.  2000.  The fate of cells in the tailbud of Xenopus laevis.. Development. 127(2):255-67.
Pfleger CM, Kirschner MW.  2000.  The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1.. Genes Dev. 14(6):655-65.
Feng Y, Olson EC, Stukenberg PT, Flanagan LA, Kirschner MW, Walsh CA.  2000.  LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome.. Neuron. 28(3):665-79.
Rohatgi R, Ho HY, Kirschner MW.  2000.  Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4, 5-bisphosphate.. J Cell Biol. 150(6):1299-310.
Kirschner MW, Gerhart J, Mitchison T.  2000.  Molecular "vitalism".. Cell. 100(1):79-88.
Luo X, Fang G, Coldiron M, Lin Y, Yu H, Kirschner MW, Wagner G.  2000.  Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20.. Nat Struct Biol. 7(3):224-9.
Yamaguchi H, Miki H, Suetsugu S, Ma L, Kirschner MW, Takenawa T.  2000.  Two tandem verprolin homology domains are necessary for a strong activation of Arp2/3 complex-induced actin polymerization and induction of microspike formation by N-WASP.. Proc Natl Acad Sci U S A. 97(23):12631-6.
1999
Fang G, Yu H, Kirschner MW.  1999.  Control of mitotic transitions by the anaphase-promoting complex.. Philos Trans R Soc Lond B Biol Sci. 354(1389):1583-90.
Kroll KL, Kirschner MW.  1999.  Easy passage: germline transgenesis in frogs.. Proc Natl Acad Sci U S A. 96(25):14189-90.
Kirschner MW.  1999.  E-publication proposal.. Science. 285(5430):1013.
Haushalter KA, Stukenberg MWTodd, Kirschner MW, Verdine GL.  1999.  Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors.. Curr Biol. 9(4):174-85.
Zou H, McGarry TJ, Bernal T, Kirschner MW.  1999.  Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis.. Science. 285(5426):418-22.
Rohatgi R, Ma L, Miki H, Lopez M, Kirchhausen T, Takenawa T, Kirschner MW.  1999.  The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly.. Cell. 97(2):221-31.
Kirschner MW.  1999.  Intracellular proteolysis.. Trends Cell Biol. 9(12):M42-5.
Odde DJ, Ma L, Briggs AH, DeMarco A, Kirschner MW.  1999.  Microtubule bending and breaking in living fibroblast cells.. J Cell Sci. 112 ( Pt 19):3283-8.
Philpott A, Tsai L, Kirschner MW.  1999.  Neuronal differentiation and patterning in Xenopus: the role of cdk5 and a novel activator xp35.2.. Dev Biol. 207(1):119-32.
Zhou BB, Kirschner MW.  1999.  Quantitative measurement of the catastrophe rate of dynamic microtubules.. Cell Motil Cytoskeleton. 43(1):43-51.
Kirschner MW.  1999.  A visit to the Hubrecht laboratory.. Int J Dev Biol. 43(7):629-31.
1998
Ma L, Rohatgi R, Kirschner MW.  1998.  The Arp2/3 complex mediates actin polymerization induced by the small GTP-binding protein Cdc42.. Proc Natl Acad Sci U S A. 95(26):15362-7.
Zhou BB, Li H, Yuan J, Kirschner MW.  1998.  Caspase-dependent activation of cyclin-dependent kinases during Fas-induced apoptosis in Jurkat cells.. Proc Natl Acad Sci U S A. 95(12):6785-90.
Fang G, Yu H, Kirschner MW.  1998.  The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation.. Genes Dev. 12(12):1871-83.
Mead PE, Zhou Y, Lustig KD, Huber TL, Kirschner MW, Zon LI.  1998.  Cloning of Mix-related homeodomain proteins using fast retrieval of gel shift activities, (FROGS), a technique for the isolation of DNA-binding proteins.. Proc Natl Acad Sci U S A. 95(19):11251-6.
Ma L, Cantley LC, Janmey PA, Kirschner MW.  1998.  Corequirement of specific phosphoinositides and small GTP-binding protein Cdc42 in inducing actin assembly in Xenopus egg extracts.. J Cell Biol. 140(5):1125-36.
Fang G, Yu H, Kirschner MW.  1998.  Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1.. Mol Cell. 2(2):163-71.
Shen M, Stukenberg PT, Kirschner MW, Lu KP.  1998.  The essential mitotic peptidyl-prolyl isomerase Pin1 binds and regulates mitosis-specific phosphoproteins.. Genes Dev. 12(5):706-20.
Kirschner MW, Gerhart J.  1998.  Evolvability.. Proc Natl Acad Sci U S A. 95(15):8420-7.
Kroll KL, Salic AN, Evans LM, Kirschner MW.  1998.  Geminin, a neuralizing molecule that demarcates the future neural plate at the onset of gastrulation.. Development. 125(16):3247-58.
McGarry TJ, Kirschner MW.  1998.  Geminin, an inhibitor of DNA replication, is degraded during mitosis.. Cell. 93(6):1043-53.
Yu H, Peters JM, King RW, Page AM, Hieter P, Kirschner MW.  1998.  Identification of a cullin homology region in a subunit of the anaphase-promoting complex.. Science. 279(5354):1219-22.
Sif S, Stukenberg PT, Kirschner MW, Kingston RE.  1998.  Mitotic inactivation of a human SWI/SNF chromatin remodeling complex.. Genes Dev. 12(18):2842-51.
1997
Yamashita YM, Peters JM, King RW, Höög C, Kirschner MW, Zachariae W, Shin TH, Galova M, Obermaier B, Nasmyth K.  1997.  BIME joins the destruction team.. Trends Cell Biol. 7(2):49.
Kothakota S, Azuma T, Reinhard C, Klippel A, Tang J, Chu K, McGarry TJ, Kirschner MW, Koths K, Kwiatkowski DJ et al..  1997.  Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis.. Science. 278(5336):294-8.
King RW, Lustig KD, Stukenberg PT, McGarry TJ, Kirschner MW.  1997.  Expression cloning in the test tube.. Science. 277(5328):973-4.
Yew PR, Kirschner MW.  1997.  Proteolysis and DNA replication: the CDC34 requirement in the Xenopus egg cell cycle.. Science. 277(5332):1672-6.
Philpott A, Porro EB, Kirschner MW, Tsai LH.  1997.  The role of cyclin-dependent kinase 5 and a novel regulatory subunit in regulating muscle differentiation and patterning.. Genes Dev. 11(11):1409-21.
Yaffe MB, Schutkowski M, Shen M, Zhou XZ, Stukenberg PT, Rahfeld JU, Xu J, Kuang J, Kirschner MW, Fischer G et al..  1997.  Sequence-specific and phosphorylation-dependent proline isomerization: a potential mitotic regulatory mechanism.. Science. 278(5345):1957-60.
Salic AN, Kroll KL, Evans LM, Kirschner MW.  1997.  Sizzled: a secreted Xwnt8 antagonist expressed in the ventral marginal zone of Xenopus embryos.. Development. 124(23):4739-48.
Lustig KD, Stukenberg PT, McGarry TJ, King RW, Cryns VL, Mead PE, Zon LI, Yuan J, Kirschner MW.  1997.  Small pool expression screening: identification of genes involved in cell cycle control, apoptosis, and early development.. Methods Enzymol. 283:83-99.
Cryns VL, Byun Y, Rana A, Mellor H, Lustig KD, Ghanem L, Parker PJ, Kirschner MW, Yuan J.  1997.  Specific proteolysis of the kinase protein kinase C-related kinase 2 by caspase-3 during apoptosis. Identification by a novel, small pool expression cloning strategy.. J Biol Chem. 272(47):29449-53.
Rankin S, Kirschner MW.  1997.  The surface contraction waves of Xenopus eggs reflect the metachronous cell-cycle state of the cytoplasm.. Curr Biol. 7(6):451-4.
Stukenberg PT, Lustig KD, McGarry TJ, King RW, Kuang J, Kirschner MW.  1997.  Systematic identification of mitotic phosphoproteins.. Curr Biol. 7(5):338-48.

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